3G2X
Structure of mimivirus NDK +Kpn - N62L double mutant complexed with dTDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2007-12-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9205 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.371, 151.492, 181.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.570 - 2.700 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b8q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.280 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 31841 | |
| <I/σ(I)> | 7.1 | 1.8 |
| Completeness [%] | 98.8 | |
| Redundancy | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | MPD 40 to 45%, Hepes 0.1M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
