3G2T
VHS Domain of human GGA1 complexed with SorLA C-terminal Phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.800, 68.300, 100.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.502 - 2.000 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jwf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.958 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.122 | 0.407 |
| Number of reflections | 26556 | |
| <I/σ(I)> | 12.84 | 3.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.05 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 18%(w/v) PEG 5000 MME, 0.2M NH4I, 0.1M TRIS-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
