3G2Q
Crystal Structure of the Glycopeptide N-methyltransferase MtfA complexed with sinefungin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.611, 71.918, 75.062 |
| Unit cell angles | 90.00, 103.82, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.180 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.25300 |
| Starting model (for MR) | 3g2m |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.437 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.260 |
| High resolution limit [Å] | 2.180 | 4.700 | 2.180 |
| Rmerge | 0.043 | 0.025 | 0.290 |
| Number of reflections | 31659 | ||
| <I/σ(I)> | 22.896 | ||
| Completeness [%] | 92.2 | 95.3 | 64.1 |
| Redundancy | 3.5 | 3.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1M MES pH 6.5, 18% PEG-monomethyl ether 5K, vapor diffusion, hanging drop, temperature 294K |
