3G2M
Crystal Structure of the Glycopeptide N-methyltransferase MtfA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-17 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.443, 71.682, 75.189 |
| Unit cell angles | 90.00, 103.02, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.239 |
| Rwork | 0.238 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.054 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.046 | 0.037 | 0.315 |
| Number of reflections | 45124 | ||
| <I/σ(I)> | 20.585 | ||
| Completeness [%] | 97.4 | 99.1 | 84.6 |
| Redundancy | 2.5 | 2.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1M MES pH 6.5, 18% PEG-monomethyl ether 5K, vapor diffusion, hanging drop, temperature 294K |
