3G29

Crystal structure of the C-terminal domain of the Rous Sarcoma Virus capsid protein: D179N mutant, neutral pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2008-10-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54178
Spacegroup name	P 43 21 2
Unit cell lengths	99.321, 99.321, 34.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.000 - 2.500
R-factor	0.26494
Rwork	0.262
R-free	0.32246
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g28
RMSD bond length	0.011
RMSD bond angle	1.251
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0044)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.394
Number of reflections	6394
<I/σ(I)>	7.8	2.2
Completeness [%]	100.0	100
Redundancy	13.7	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.9	291	0.20M Tris/HCl, pH7.9, 1.5M Ammonium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K