3G27
Structure of a putative bacteriophage protein from Escherichia coli str. K-12 substr. MG1655
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-24 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.311, 71.311, 32.333 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.880 - 2.100 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.24900 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.403 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.065 | 0.226 |
| Number of reflections | 6049 | |
| <I/σ(I)> | 48.638 | |
| Completeness [%] | 98.6 | 87.7 |
| Redundancy | 9.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.2M CaCl2, 20%PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
