3G25
1.9 Angstrom Crystal Structure of Glycerol Kinase (glpK) from Staphylococcus aureus in Complex with Glycerol.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.98400 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.750, 193.742, 91.669 |
| Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
| Resolution | 44.810 - 1.900 |
| R-factor | 0.19529 |
| Rwork | 0.193 |
| R-free | 0.24562 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ezw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.679 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0051) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.081 | 0.494 |
| Number of reflections | 168279 | |
| <I/σ(I)> | 15.6 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 1:1 v/v protein solution [10mg/ml protein, 0.3M NaCl, 10mM Na Hepes pH 7.5), screen solution (0.2M Na dihydrogen Phosphate , 20% PEG 3350, 6% Glycerol), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
