3G20
Crystal structure of the major pseudopilin from the type 2 secretion system of enterohaemorrhagic Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-30 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.120, 36.830, 61.270 |
| Unit cell angles | 106.94, 99.05, 90.17 |
Refinement procedure
| Resolution | 35.010 - 1.780 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fu1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.010 | 35.010 | 1.820 |
| High resolution limit [Å] | 1.780 | 7.950 | 1.780 |
| Rmerge | 0.064 | 0.017 | 0.545 |
| Number of reflections | 39113 | 438 | 2732 |
| <I/σ(I)> | 44.8 | 1.8 | |
| Completeness [%] | 94.2 | 94.4 | 88.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 1.0M Na citrate, 0.1M CHES pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
