3G1P
Crystals structure of PhnP from E.coli K-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-30 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.650, 75.405, 83.228 |
| Unit cell angles | 90.00, 126.33, 90.00 |
Refinement procedure
| Resolution | 67.000 - 1.400 |
| R-factor | 0.18705 |
| Rwork | 0.186 |
| R-free | 0.20982 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.316 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.120 | 1.460 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 109380 | |
| <I/σ(I)> | 31 | 5.5 |
| Completeness [%] | 94.4 | 64.9 |
| Redundancy | 7.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | MES, Tris, S-Malate, PEG 8000, PEG 400, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
