3G1K
Mth0212 (WT) crystallized in a monoclinic space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8148 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.635, 80.345, 81.839 |
| Unit cell angles | 90.00, 90.32, 90.00 |
Refinement procedure
| Resolution | 39.280 - 3.100 |
| R-factor | 0.20605 |
| Rwork | 0.203 |
| R-free | 0.26604 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fzi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.139 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 10535 | |
| <I/σ(I)> | 9.8 | 4 |
| Completeness [%] | 99.0 | 94.1 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | reservoir: 10% PEG, 100mM sodium cacodylate pH 6.5; protein solution: 1mM MgCl2, 50mM KCl, 10mM KH2PO4/K2HPO4 pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
