3G1J
Structure from the mobile metagenome of Vibrio cholerae. Integron cassette protein VCH_CASS4.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-24 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 62 |
| Unit cell lengths | 121.043, 121.043, 27.014 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.660 - 1.700 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.21890 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.668 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.041 | 0.615 |
| Number of reflections | 25217 | |
| <I/σ(I)> | 37.14 | 2.2 |
| Completeness [%] | 91.7 | 100 |
| Redundancy | 6.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 296 | 0.1M Na(OAC), 0.2M NaFormate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
