3G1I

Crystal structure of the C-terminal domain of the Rous Sarcoma Virus capsid protein: Intermediate pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2007-10-11
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54178
Spacegroup name	I 41
Unit cell lengths	107.164, 107.164, 35.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.890 - 2.100
R-factor	0.19668
Rwork	0.194
R-free	0.24535
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.261
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0044)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.067	0.323
Number of reflections	11888
<I/σ(I)>	10.4	3
Completeness [%]	100.0	100
Redundancy	14.4	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.2M Bis-tris propane/HCl, pH8.5, 0.75-1.85M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K