3G1E

X-ray crystal structure of coil 1A of human vimentin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-10
Detector	OXFORD ONYX CCD
Spacegroup name	P 41 21 2
Unit cell lengths	35.496, 35.496, 108.022
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.650 - 1.830
Rwork	0.253
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DIMERIC MODEL BUILT FROM PDB ENTRIES 1GK7 AND 1D7M
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	CrysalisPro (PRO (OXFORD DIFFRACTION))
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	11.650	1.860
High resolution limit [Å]	1.770	1.770
Rmerge	0.099	0.414
Number of reflections	6364
<I/σ(I)>	7	1.6
Completeness [%]	87.5	43.8
Redundancy	9.3	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6	298	20 % PEG, 33 % isopropanol, 0.1 M trisodium citrate, pH 5.6, vapour diffusion, temperature 298K