3G1C
The crystal structure of a TrpR like protein from Eubacterium eligens ATCC 27750
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 48.788, 48.788, 126.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.630 - 2.200 |
| R-factor | 0.20632 |
| Rwork | 0.205 |
| R-free | 0.23657 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.630 | 2.257 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.106 | 0.548 |
| Number of reflections | 7885 | |
| <I/σ(I)> | 31.34 | 1.28 |
| Completeness [%] | 99.2 | 94.44 |
| Redundancy | 15.4 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M HEPES pH7.5, 0.5M Magnesium formate dihydrate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
