3G18

Crystal structure of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-31
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 41
Unit cell lengths	56.847, 56.847, 125.545
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.600
R-factor	0.229
Rwork	0.227
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dv7
RMSD bond length	0.005
RMSD bond angle	1.430
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.600
Rmerge	0.048
Number of reflections	52290
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	30% PEG ME 550, 0.1M hepes, 0.05M magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K