3G16

Crystal structure of protein of unknown function with cystatin-like fold (YP_001022489.1) from METHYLOBIUM PETROLEOPHILUM PM1 at 1.45 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-12
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97864,0.97817
Spacegroup name	P 21 21 21
Unit cell lengths	48.560, 68.450, 107.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.973 - 1.450
R-factor	0.149
Rwork	0.148
R-free	0.16700
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.664
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.973	27.973	1.500
High resolution limit [Å]	1.450	3.120	1.450
Rmerge	0.025	0.014	0.374
Number of reflections	63677	12025	10613
<I/σ(I)>	16.47	47.2	2
Completeness [%]	97.7	97.1	88.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	1.0000M LiCl, 20.0000% PEG-6000, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K