3G0V
Crystal structure of the C-terminal domain from the Rous Sarcoma Virus capsid protein: mutant D179A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.763, 76.708, 32.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.000 - 2.000 |
| R-factor | 0.19815 |
| Rwork | 0.197 |
| R-free | 0.22842 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g28 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.368 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.078 | 0.333 |
| Number of reflections | 6413 | |
| <I/σ(I)> | 9.2 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 291 | 0.2M Succinic acid/KOH, pH4.3, 24% PEG8000, 1M Sodium Nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
