3G0Q
Crystal Structure of MutY bound to its inhibitor DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-10 |
| Detector | RIGAKU RAXIS V |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.700, 85.900, 142.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.27700 |
| Starting model (for MR) | 1rrq |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.088 | 0.062 | 0.472 |
| Number of reflections | 24152 | ||
| <I/σ(I)> | 39.75 | ||
| Completeness [%] | 99.9 | 99.2 | 100 |
| Redundancy | 6.8 | 5.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | Tris, PEG4000 calcium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris | ||
| 2 | 1 | 1 | PEG4000 | ||
| 3 | 1 | 1 | Calcium acetate | ||
| 4 | 1 | 2 | Tris | ||
| 5 | 1 | 2 | PEG4000 | ||
| 6 | 1 | 2 | Calcium acetate |
