3G0M
Crystal structure of cysteine desulfuration protein SufE from Salmonella typhimurium LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.791, 42.617, 45.006 |
| Unit cell angles | 90.00, 98.15, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.760 |
| R-factor | 0.18774 |
| Rwork | 0.185 |
| R-free | 0.23972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mzg |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.638 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.042 | 0.293 |
| Number of reflections | 11678 | |
| <I/σ(I)> | 31.6 | 3.1 |
| Completeness [%] | 97.6 | 79.1 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 30% PEG 400, 0.1M Li2SO4, 0.1M Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
