3G05
Crystal structure of N-terminal domain (2-550) of E.coli MnmG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 144.593, 144.593, 271.019 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.490 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ces |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.333 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.610 |
| High resolution limit [Å] | 3.490 | 7.510 | 3.490 |
| Rmerge | 0.108 | 0.031 | 0.640 |
| Number of reflections | 42430 | ||
| <I/σ(I)> | 16.462 | ||
| Completeness [%] | 99.8 | 99.9 | 98.5 |
| Redundancy | 7.8 | 9.8 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 1.3M Lithium sulfate, 0.1M Tris-HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
