3FZU
IgG1 Fab characterized by H/D exchange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 243.051, 61.938, 100.846 |
| Unit cell angles | 90.00, 113.94, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.21301 |
| Rwork | 0.210 |
| R-free | 0.26107 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.743 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.089 |
| Number of reflections | 42711 |
| <I/σ(I)> | 31.7 |
| Completeness [%] | 93.9 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 38% PEG 400, 200 mM sodium chloride, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
