3FZG
Structure of the 16S rRNA methylase ArmA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.110, 90.080, 65.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.040 - 2.000 |
| R-factor | 0.1899 |
| Rwork | 0.190 |
| R-free | 0.20630 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.040 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.022 | 0.071 |
| Number of reflections | 15048 | |
| <I/σ(I)> | 41.38 | 17.76 |
| Completeness [%] | 98.6 | 98.1 |
| Redundancy | 3.6 | 3.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG 3350, 50mM bis-tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
