3FZE

Structure of the 'minimal scaffold' (ms) domain of Ste5 that cocatalyzes Fus3 phosphorylation by Ste7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-03
Detector	ADSC QUANTUM Q315r
Wavelength(s)	1.11587
Spacegroup name	P 21 21 21
Unit cell lengths	46.803, 63.569, 69.091
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.165 - 1.601
R-factor	0.2101
Rwork	0.209
R-free	0.23670
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.952
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	25696
<I/σ(I)>	19.3	4
Completeness [%]	92.4	93.9
Redundancy	4.3	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	Mixed 10mg/mL protein 1:1 (vol:vol) with solution of 20% PEG3350, 0.1M citrate pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	Mixed 10mg/mL protein 1:1 (vol:vol) with solution of 20% PEG3350, 0.1M citrate pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K