3FZB
Crystal structure of the tail terminator protein from phage lambda (gpU-WT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-18 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.100, 127.990, 82.910 |
| Unit cell angles | 90.00, 108.92, 90.00 |
Refinement procedure
| Resolution | 70.100 - 2.800 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.100 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.099 | 0.312 |
| Number of reflections | 36045 | |
| <I/σ(I)> | 8.6 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.93 | 3.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2M Tri-sodium citrate, 2.2M Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
