3FYS
Crystal Structure of DegV, a fatty acid binding protein from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 2.2909 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.100, 84.700, 42.200 |
| Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
| Resolution | 11.953 - 2.500 |
| R-factor | 0.1523 |
| Rwork | 0.148 |
| R-free | 0.22460 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.900 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 9588 | |
| <I/σ(I)> | 60.25 | 31.6 |
| Completeness [%] | 93.5 | 65.3 |
| Redundancy | 3.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.15M potassium bromide, 30% w/v polyethylene glycol monomethyl ether 2000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
