3FYQ
Structure of Drosophila melanogaster talin IBS2 domain (residues 1981-2168)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2008-12-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9808, 0.9811, 0.9694 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.919, 40.079, 57.547 |
| Unit cell angles | 90.00, 112.87, 90.00 |
Refinement procedure
| Resolution | 36.150 - 1.950 |
| R-factor | 0.20875 |
| Rwork | 0.206 |
| R-free | 0.25548 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.740 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 13427 | |
| Completeness [%] | 92.2 | 54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.95 | 294 | 0.1M Bicine pH 9.95, 1-2M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
