3FYL
GR DNA binding domain:CGT complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-24 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.115889 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.445, 37.770, 96.679 |
Unit cell angles | 90.00, 123.50, 90.00 |
Refinement procedure
Resolution | 35.240 - 1.630 |
R-factor | 0.1979 |
Rwork | 0.196 |
R-free | 0.23370 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.538 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.690 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.052 | 0.534 |
Number of reflections | 31824 | |
<I/σ(I)> | 19.5 | 1.4 |
Completeness [%] | 71.4 | 12.1 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 50mM Tris, pH 8.5, 100mM KCl, 10mM MgCL2, 30% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Tris | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 2 | Tris | ||
5 | 1 | 2 | KCl | ||
6 | 1 | 2 | MgCl2 | ||
7 | 1 | 2 | PEG 400 |