3FYB
Crystal structure of a protein of unknown function (DUF1244) from Alcanivorax borkumensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.501, 69.536, 81.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.190 - 1.800 |
| R-factor | 0.1888 |
| Rwork | 0.187 |
| R-free | 0.22147 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o35 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.374 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.190 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.058 | 0.392 |
| Number of reflections | 22091 | |
| <I/σ(I)> | 29.526 | 5.3 |
| Completeness [%] | 99.6 | 95.5 |
| Redundancy | 6.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.2M CaCl2, 20% PEG 3350. 25% Ethylene glycol added as a cryoprotectant, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
