3FY3
Crystal structure of truncated hemolysin A from P. mirabilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-02 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.273, 119.528, 34.199 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.970 - 1.800 |
| R-factor | 0.15063 |
| Rwork | 0.149 |
| R-free | 0.20283 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rwr |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.745 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.092 | 0.503 |
| Number of reflections | 22150 | |
| <I/σ(I)> | 13.9 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.4 | 17.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 286 | 0.8 M Na/KH2PO4, 0.1 M HEPES pH 7.5 , VAPOR DIFFUSION, SITTING DROP, temperature 286K |
