3FXB
Crystal structure of the ectoine-binding protein UehA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.000, 61.300, 86.300 |
| Unit cell angles | 90.00, 102.60, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.900 |
| R-factor | 0.22009 |
| Rwork | 0.218 |
| R-free | 0.26161 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vpo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.111 | 0.426 |
| Number of reflections | 14412 | |
| <I/σ(I)> | 11.32 | 3.5 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 100mM Na Citrate pH 5.6-6.0, 2.4-2.6M Ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
