3FWR
Crystal Structure of the CBS domains from the Bacillus subtilis CcpN repressor complexed with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93300 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.273, 103.047, 98.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.640 - 2.450 |
| R-factor | 0.20219 |
| Rwork | 0.200 |
| R-free | 0.28073 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fv6 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.872 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.530 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.103 | 0.545 |
| Number of reflections | 9555 | |
| <I/σ(I)> | 19.5 | 2.6 |
| Completeness [%] | 92.9 | 92.9 |
| Redundancy | 6.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 17% PEG 2000 MME, 0.1M HEPES, 8mg/ml Protein; Drops 1+1 microliter, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
