3FW6
Crystal structure of CelM2, a bifunctional glucanase-xylanase protein from a metagenome library
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.663, 86.952, 111.285 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.260 - 2.300 |
| R-factor | 0.2073 |
| Rwork | 0.207 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eqd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.137 | 0.332 |
| Total number of observations | 219990 | |
| Number of reflections | 37615 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 5.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293.5 | 0.1M MES pH 5.9, 10% (v/v) PEG 3350, 2% ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293.5K |
