3FVZ
Structure of Peptidyl-alpha-hydroxyglycine alpha-Amidating Lyase (PAL)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-18 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.28241 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.176, 75.056, 97.474 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.220 - 2.350 |
| R-factor | 0.20615 |
| Rwork | 0.203 |
| R-free | 0.26333 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.215 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.220 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 16524 | |
| <I/σ(I)> | 60.9 | 4.2 |
| Completeness [%] | 99.7 | 96.9 |
| Redundancy | 11.3 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 0.1M sodium acetate pH=4.8, 0.2mM zinc(II) acetate, 0.2mM iron(III) chloride - 0.2ml mother liquor in reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
