3FVW

Crystal structure of the Q8DWD8_STRMU protein from Streptococcus mutans. Northeast Structural Genomics Consortium target SmR99.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-01-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97862, 0.97922, 0.91837,
Spacegroup name	P 32 2 1
Unit cell lengths	99.297, 99.297, 90.114
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.188
R-free	0.21670
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.305
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX (D/E)
Refinement software	CNS (1.2 (using xtal_twin utilities))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.134	0.786
Number of reflections	44160
<I/σ(I)>	15.9	2.03
Completeness [%]	100.0	100
Redundancy	7.7	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	291	18-20% PEG 3350, 0.2M DL-Malic acid, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K