3FVI
Crystal Structure of Complex of Phospholipase A2 with Octyl Sulfates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.988, 82.061, 123.824 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.540 - 2.700 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.540 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.119 | 0.466 |
| Number of reflections | 18967 | |
| <I/σ(I)> | 15.3 | 3.1 |
| Completeness [%] | 99.1 | 98.1 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 0.1 M sodium acetate, 8% w/v PEG 8000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
