3FVH
Polo-like kinase 1 Polo box domain in complex with Ac-LHSpTA-NH2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.310, 62.412, 46.607 |
| Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
| Resolution | 24.171 - 1.580 |
| R-factor | 0.1942 |
| Rwork | 0.192 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1umw |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 28949 | |
| <I/σ(I)> | 17.4 | 8.6 |
| Completeness [%] | 96.4 | 94.2 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 2000 MME, Tris pH 8.5, trimethyl-amine oxide, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
