3FVD
Crystal structure of a member of enolase superfamily from ROSEOVARIUS NUBINHIBENS ISM complexed with magnesium
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 126.518, 126.518, 97.294 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.300 |
| R-factor | 0.167 |
| Rwork | 0.164 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.827 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.294 | 33.010 | 2.340 |
| High resolution limit [Å] | 2.220 | 7.020 | 2.220 |
| Rmerge | 0.170 | 0.081 | 0.954 |
| Total number of observations | 35441 | 133456 | |
| Number of reflections | 39470 | ||
| <I/σ(I)> | 3.708 | 7.6 | 0.7 |
| Completeness [%] | 99.6 | 99.1 | 97.3 |
| Redundancy | 28.5 | 25.4 | 24.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 5M Sodium formate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
