3FUV
Apo-form of T. thermophilus 16S rRNA A1518 and A1519 methyltransferase (KsgA) in space group P43212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.111, 85.111, 215.867 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| R-factor | 0.233 |
| Rwork | 0.229 |
| R-free | 0.30900 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.096 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.086 | 0.420 |
| Number of reflections | 59133 | |
| <I/σ(I)> | 29.8 | 3.36 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch technique under oil | 8.5 | 277 | 160 mM magnesium chloride hexahydrate, 80 mM TRIS (pH 8.5), 24% w/v polyethylene glycol 4000 and 20% v/v anhydrous glycerol, microbatch technique under oil, temperature 277K |
