3FUQ
Glycosylated SV2 and Gangliosides as Dual Receptors for Botulinum Neurotoxin Serotype F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.330, 74.180, 74.770 |
| Unit cell angles | 90.00, 106.36, 90.00 |
Refinement procedure
| Resolution | 29.170 - 2.100 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fuo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MERLOT |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.170 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 23953 | |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 91.8 | 58 |
| Redundancy | 6.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1 M Tris-HCL, 18% ethanol and 150 mM NaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
