3FU8
Melanocarpus albomyces laccase crystal soaked (10 sec) with 2,6-dimethoxyphenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.842 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.710, 62.370, 125.140 |
| Unit cell angles | 90.00, 96.36, 90.00 |
Refinement procedure
| Resolution | 19.780 - 1.800 |
| R-factor | 0.20595 |
| Rwork | 0.203 |
| R-free | 0.25555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.638 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 122633 | |
| <I/σ(I)> | 16.76 | 4.9 |
| Completeness [%] | 98.6 | 95.2 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 15 % PMME 2000, 0,2 M ammonium sulphate, 0,1 M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
