3FU7
Melanocarpus albomyces laccase crystal soaked (4 sec) with 2,6-dimethoxyphenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.365 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.120, 62.100, 124.630 |
| Unit cell angles | 90.00, 96.36, 90.00 |
Refinement procedure
| Resolution | 19.610 - 1.670 |
| R-factor | 0.19038 |
| Rwork | 0.189 |
| R-free | 0.22033 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q9o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.407 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Number of reflections | 149347 | |
| <I/σ(I)> | 11.5 | 4.2 |
| Completeness [%] | 97.2 | 77.5 |
| Redundancy | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 15 % PMME 2000, 0,2 M ammonium sulphate, 0,1 M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
