3FTM
Class II ligase ribozyme product-template duplex, structure 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 31.550, 31.550, 171.066 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 2.700 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26800 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.046 | 0.442 |
| Number of reflections | 4565 | |
| <I/σ(I)> | 29.4 | 2.9 |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.2 mM RNA duplex, 10mM magnesium chloride, 200mM magnesium acetate, 15% PEG 8000, 50mM cacodylate pH 6.5 , VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | magnesium chloride | ||
| 2 | 1 | 1 | magnesium acetate | ||
| 3 | 1 | 1 | PEG 8000 | ||
| 4 | 1 | 1 | cacodylate | ||
| 5 | 1 | 2 | magnesium chloride | ||
| 6 | 1 | 2 | magnesium acetate | ||
| 7 | 1 | 2 | PEG 8000 | ||
| 8 | 1 | 2 | cacodylate |
