3FTB
The crystal structure of the histidinol-phosphate aminotransferase from Clostridium acetobutylicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.556, 121.663, 94.185 |
Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
Resolution | 37.248 - 2.000 |
R-factor | 0.1764 |
Rwork | 0.174 |
R-free | 0.21230 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.008 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | CNS |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.250 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.113 | 0.500 |
Number of reflections | 178715 | |
<I/σ(I)> | 25.2 | 1.89 |
Completeness [%] | 89.4 | 62.45 |
Redundancy | 8.8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 20% Ethanol, 0.1M Tris , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 250K, temperature 298K |