3FSW
Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.686, 56.700, 58.242 |
| Unit cell angles | 111.96, 95.38, 105.12 |
Refinement procedure
| Resolution | 49.800 - 2.000 |
| R-factor | 0.19267 |
| Rwork | 0.189 |
| R-free | 0.25573 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fs9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.131 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | 0.266 |
| Number of reflections | 25606 | |
| <I/σ(I)> | 11.9 | 5.4 |
| Completeness [%] | 91.7 | 81.5 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM Hepes pH 8.0, 34% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
