3FSV
Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAHAAAM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | ADSC QUANTUM Q315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.836, 55.819, 74.482 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.680 - 2.300 |
| R-factor | 0.26207 |
| Rwork | 0.257 |
| R-free | 0.30839 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fs9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.969 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.103 | 0.373 |
| Number of reflections | 5240 | |
| <I/σ(I)> | 21.8 | 7.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 150mM potassium bromide, 30% PEG MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
