3FSN
Crystal structure of RPE65 at 2.14 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03324 |
| Spacegroup name | P 65 |
| Unit cell lengths | 176.516, 176.516, 86.862 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.220 - 2.140 |
| R-factor | 0.1816 |
| Rwork | 0.180 |
| R-free | 0.21635 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.404 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 83509 | |
| <I/σ(I)> | 14.2 | 2.16 |
| Completeness [%] | 99.5 | 98.3 |
| Redundancy | 6.3 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | precipitant: PEG200, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
