3FSH
Crystal structure of the ubiquitin conjugating enzyme Ube2g2 bound to the G2BR domain of ubiquitin ligase gp78
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-27 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.719, 105.719, 142.855 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.760 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cyx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.860 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Number of reflections | 21654 | |
| <I/σ(I)> | 19.4 | 2.1 |
| Completeness [%] | 99.5 | 95.5 |
| Redundancy | 8.4 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | 2.8M SODIUM FORMATE, 0.1M TRIS PH 7.7, 5MM DTT, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
