3FRX
Crystal Structure of the Yeast Orthologue of RACK1, Asc1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Detector technology | CCD |
| Collection date | 2007-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793713 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.660, 85.410, 119.100 |
| Unit cell angles | 90.00, 134.80, 90.00 |
Refinement procedure
| Resolution | 17.519 - 2.130 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.519 | 2.157 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 125144 | |
| Completeness [%] | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 13.3% PEG 2000 MME, 22 mM MnOAc, 100 mM NaOAc, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
