3FRN
CRYSTAL STRUCTURE OF flagellar protein FlgA FROM Thermotoga maritima MSB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-05-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.087, 28.631, 95.530 |
| Unit cell angles | 90.00, 106.83, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.24234 |
| Rwork | 0.241 |
| R-free | 0.28198 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.026 | 2.020 |
| Rmerge | 0.089 | 0.650 |
| Number of reflections | 18429 | |
| <I/σ(I)> | 8 | 1.6 |
| Completeness [%] | 97.8 | 63 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 200MM AMMONIUM PHOSPHATE, PH 7.5, 20% PEG3350, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
