3FRL
The 2.25 A crystal structure of LipL32, the major surface antigen of Leptospira interrogans serovar Copenhageni
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.459, 0.97814, 0.97831 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 126.725, 126.725, 95.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 2.250 |
| R-factor | 0.18358 |
| Rwork | 0.181 |
| R-free | 0.22712 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.581 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.071 | 0.466 |
| Number of reflections | 42419 | |
| <I/σ(I)> | 22 | 1.9 |
| Completeness [%] | 99.4 | 95.8 |
| Redundancy | 10.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 289 | 1.4 M trisodium citrate, 0.1 M HEPES, 20 mM triethanolamine-HCl, pH 7.7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
